"This extremely well written and useful collection of laboratory methods for the study of steroid biology is one of a series in Methods in Molecular Biology devoted to steroid hormones and their receptors. The book provides an up-to-date compilation of protocols and assays that are important for the study of steroids and their receptors... it will be invaluable to researchers already in the field to compare protocols and improve methods...The book is divided into sections of bioinformatics, purification protocols, steroid hormone binding assays, protein interaction assays, and cancer research and drug discovery. The bioinformatics section leads off the book with a wealth of information on web sites that are relevant for steroid hormones and their receptors. While there are probably over 40 different proteins involved in steroid hormone receptor interaction, the section on protein interactions is superb. It actually clarifies a good part of the literature as so many of the proteins have different names for the same molecule. Understanding the mechanisms of isolation of these proteins puts the literature into perspective. Furthermore, the protocols are in sufficient detail that our lab was able to adapt several of the techniques immediately." -Doody's Health Sciences Book Review Journal
"This volume, part of the series 'Methods in Molecular Biology', describes in detail the protocols and assays used in the study of steroid hormone receptors." - Journal of Pediatric Endocrinology and Metabolism
"Timely and highly accessible, Steroid Receptor Methods. Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery." - Biomedicine & Pharmacotherapy
"Anyone working techniques, or who is tempted to enter the field, would be well advised to obtain this book and read the relevant chapters first. PhD student and established scientist alike will find Steroid Receptor Methods a valuable addition the the library." - Molecular Biotechnology
"Overall, the book is well written by an impressive list of experts and represents a valuable resource for both inexperienced and seasoned researchers of steroid receptors." -Today's Life Science
Once we have added in the necessary testosterone, the next step is controlling the impending estrogen and progesterone activity that will result from the use of the Nandrolone hormone. This makes the use of anti- estrogens highly recommended; most will need an Aromatase Inhibitor (AI). However, if we suppress estrogenic activity too much, this can lead to complications; it can lead to sexually related side effects as well as damage cholesterol. Conversely, if we do not control estrogen and let the hormone run wild, high levels of estrogen can lead to gynecomastia , water retention and also negatively affect sexual function. Find the right balance of testosterone to estrogen and you will not have a problem when stacking Deca Durabolin.
As demonstrated in progesterone receptor-deficient mice, the physiological effects of progesterone depend completely on the presence of the human progesterone receptor (hPR), a member of the steroid-receptor superfamily of nuclear receptors. The single-copy human (hPR) gene uses separate promoters and translational start sites to produce two isoforms, hPR-A and -B, which are identical except for an additional 165 amino acids present only in the N terminus of hPR-B.  Although hPR-B shares many important structural domains with hPR-A, they are in fact two functionally distinct transcription factors, mediating their own response genes and physiological effects with little overlap. Selective ablation of PR-A in a mouse model, resulting in exclusive production of PR-B, unexpectedly revealed that PR-B contributes to, rather than inhibits, epithelial cell proliferation both in response to estrogen alone and in the presence of progesterone and estrogen. These results suggest that in the uterus, the PR-A isoform is necessary to oppose estrogen-induced proliferation as well as PR-B-dependent proliferation.